Example I_to,f model with 6 free rate constants

A) Topology of the model B) Experimental data points and simulated fits for conductance voltage and steady state inactivation protocols. Purple validation points indicate points added in the simulation to calculate a model’s validation error when checking for overfitting. C) Experimental recovery from inactivation and simulated fit with added validation points to compute validation error for overfitting prevention. D) Normalized experimental current traces at 20 mV and 60 mV (black) with corresponding simulated traces. In purple is the experimental 40 mV normalized current trace along with its associated fit for validation. E) I_to,f current trace when included in the Tor-Ord [50] human ventricular action potential model (F) All experimental data are from Johnson et al. [60] Experimental data points (black) are mean ± SEM.

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