Estimated gene-to-trait effects for TWMR, PTWAS, and MRLocus on five eQTL and GWAS datasets for strong candidate genes for mediation of the GWAS trait and one auxiliary tissue example (LIPC in blood).

80% confidence or credible intervals are shown (MRLocus provides quantile-based credible intervals). The artery eQTL were estimated from inverse normal transformed expression data from GTEx, transformed z-scores for eQTLGen, while the liver meta-analysis eQTL were estimated from log2 expression data, thus the estimated slopes represent changes in SD of artery or blood gene expression on log odds for CAD risk and on SDs of lipid levels, and doubling of liver gene expression on SDs of lipid levels.