Essential roles of VPS34/VPS15 PI3 kinase complex in Yl recycling and yolk granule biogenesis.

Confocal fluorescent microscopy and DIC imaging of stage 10 egg chambers from flies carrying genome-tagging Yl-eGFP-3xHA with oocyte-specific expression of (A-C, G, H) control firefly Luciferase RNAi or (D-F, I, J) dsRNA again VPS34/PI3K59F, co-labeled with antibodies against GFP (green) and (A-F) lysotracker (red), or (G-J) endogenous Rab7 (red) and F-Actin (blue), as annotated. (B, E, H, J) High-magnification view of the cortex regions highlighted in (A, D, G, I), respectively. (C, F) Zoom-in view of the areas highlighted in (B, E), respectively. Images are presented as overlaying images in color or as individual channels in gray, as annotated. Genotypes: The samples were from adult females flies heterozygous for Yl-eGFP-3xHA reporter (p{mini-W+, yl-eGFP-3xHA}) and matalpha4-GAL-VP16 (BDSC #7062) driver together with (A-C, G, H). P{TRiP.JF01355}attP2 (BDSC#31603) or (D-F, I, J) P{TRiP.HMJ30324}attP40 (BDSC #64011). The sizes of the scales as annotated inside images.

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