ppat.1009543.s004.tif (5.41 MB)

Epitope comparison between the autologous mAbs antibodies and the broadly neutralizing antibodies.

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posted on 2021-09-24, 18:00 authored by Safia S. Aljedani, Tyler J. Liban, Karen Tran, Ganesh Phad, Suruchi Singh, Viktoriya Dubrovskaya, Pradeepa Pushparaj, Paola Martinez-Murillo, Justas Rodarte, Alex Mileant, Vidya Mangala Prasad, Rachel Kinzelman, Sijy O’Dell, John R. Mascola, Kelly K. Lee, Gunilla B. Karlsson Hedestam, Richard T. Wyatt, Marie Pancera

(A) Side and top view surface representation of 16055 NFL (PDB:5UM8) color-coded and labeled as mentioned earlier (Figs 3 and 7). Epitopes targeted by the bNAbs PGT145 (Heavy chain, deepteal; Light chain, light teal)(PDB:5V8L), PG9 (Heavy chain, tv orange; Light chain, wheat)(PDB: 3U2S), PTG122 (Heavy chain, dark gray; Light chain, light gray) and VRC01 (Heavy chain, firebrick; Light chain, light firebrick) (PDB: 5FYK) and our Autologous mAbs (D11A.B5, D15.SD7, D19.PA8, and VD20.5A4) are highlighted and color coded. (B) Side view and (C) Top view superpositions of the bNAbs antibodies structures of PGT145, PG9, PTG122 and VRC01 onto the 16055 NFL trimer, showing how they target their epitopes. Trimer and mAbs are shown in surface representation.

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