figshare
Browse
ppat.1009280.s001.tif (8.25 MB)

Enrichment of bacterial RNA from infected cells.

Download (8.25 MB)
figure
posted on 2021-08-30, 17:33 authored by TuShun R. Powers, Amanda L. Haeberle, Alexander V. Predeus, Disa L. Hammarlöf, Jennifer A. Cundiff, Zeus Saldaña-Ahuactzi, Karsten Hokamp, Jay C. D. Hinton, Leigh A. Knodler

Total RNA was extracted from HeLa epithelial cells (HeLa), wild-type S. Typhimurium SL1344 grown to late log-phase in LB-Miller broth (STm), S. Typhimurium isolated from Earle’s balanced salt solution (EBSS)-treated cells at 8 h p.i. or S. Typhimurium isolated from wortmannin (WTM)-treated cells at 8 h p.i. RNA quality was analyzed by electrophoretic separation using an Agilent Bioanalyzer 2100. Ladder sizes shown in kb.

(TIF)

History

Usage metrics

    PLOS Pathogens

    Categories

    Keywords

    showed increased expressionremain largely unknownphysicochemical conditions encounteredpathogenicity island 1cell microscopic analysiscauses diarrheal diseaseparallel colonization strategiesepithelial cell lysisserovar typhimurium (<scv ), followedinduced expression signatures>- containing vacuoleidentified bacterial genesbacterial genes requiredsalmonella enterica bacterial releasesalmonella epithelial cellsvirulence genescytosolic colonizationzoonotic pathogenunusual lifestyletypically associatedtyphimurium withintyphimurium replicationtwo nichesuccessfully colonizestudy revealssoxs sita seq approachpermissive environmentoxidative stressmnth manganese deprivationmagnesium deprivationintracellular nichegastrointestinal tractfepb extracellular bacteriaepithelial cytosolenvironmental biosensorsentc begins withinbased rnaarchetypical vacuole

    Licence

    CC BY 4.0

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC