pone.0247375.g001.tif (204.15 kB)
Efficacy of different sgRNAs against B2M in ablating cell surface MHC-I antigen.
figure
posted on 2021-03-04, 18:49 authored by Michael Hansen, Xiaopin Cai, Sara Bowen, David A. Largaespada, Ming V. LiHEK293T cells were transfected with combination of pCMV-spCas9 and different sgRNAs against B2M (B2M1, C; B2M2, D; and B2M3, E), at a ratio of 1:1.5 for Cas9 and sgRNA plasmid. 5 days after transfection, they were stained with FITC-conjugated antibody against human HLA-A,B&C and run on flow cytometer. A and B were negative (no antibody staining) and positive (wild-type HEK293T cells stained with antibody) controls, respectively.
