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Effects of IL-18 on osteoclast formation

figure

posted on 2011-12-30, 13:58 authored by Nobuyuki Udagawa, Shigeru Kotake, Naoyuki Kamatani, Naoyuki Takahashi, Tatsuo Suda

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Taken from "The molecular mechanism of osteoclastogenesis in rheumatoid arthritis"

Arthritis Research 2002;4(5):281-289.

Published online 12 Apr 2002

PMCID:PMC128939.

Mouse spleen cells and osteoblasts from wild-type mice (WT), IFN-γ receptor type II-knockout mice (IFNγR KO) or granulocyte–macrophage colony-stimulating factor-knockout mice (GM-CSF KO) were cocultured with 1-α,25(OH)D and prostaglandin E (PGE) in the presence or absence of IL-18. In some cocultures, T-cell-depleted spleen cells and osteoblasts were cocultured in the presence and absence of WT T cells or GM-CSF KO T cells. In each coculture, numbers of tartrate-resistant acid phosphatase-positive osteoclasts formed were scored.