Effects of Ac3IV treatment on pancreatic morphology in STZ-diabetic GluCreERT2;Rosa26-eYFP mice.

Parameters were assessed in the head (A,C,E,G) and tail (B,D,F,H) of the pancreas following 12 days twice-daily treatment with saline (0.9% NaCl) vehicle or Ac3IV (25 nmol/kg bw, i.p.) in STZ-diabetic GluCreERT2;Rosa26-eYFP transgenic mice. Islet (A,B), beta- (C,D) and alpha-cell (E,F) areas were assessed using CellF imaging software and the closed loop polygon tool. Islet size distributions in the head (G) and tail (H) of the pancreas are also shown. Representative images (40X) of stained islets are provided in panels I and J. Values are mean ± SEM for n = 6 mice. *p<0.05, **p<0.01, ***p<0.001 compared to saline vehicle. Δp<0.05, ΔΔp<0.01 and ΔΔΔp<0.001 compared to STZ-diabetic controls. ND—not detected.