pone.0246647.s007.tif (2 MB)
Effect of uncrosslinking time on hot-start Taq activity.
figure
posted on 2021-02-03, 18:59 authored by Thomas G. W. Graham, Claire Dugast-Darzacq, Gina M. Dailey, Xammy H. Nguyenla, Erik Van Dis, Meagan N. Esbin, Abrar Abidi, Sarah A. Stanley, Xavier Darzacq, Robert TjianBEARmix reactions prepared with hot-start Taq (hsTaq) were incubated for 10, 15, or 20 min at 95°C to reverse formaldehyde crosslinks prior to amplification cycles. A 5 min incubation at 95°C was used for regular (non-crosslinked) Taq. Thin curves represent traces for 7 individual reactions, while thick curves represent their average. Longer uncrosslinking times led to earlier amplification, however amplification with hot-start Taq was still delayed relative to unmodified Taq.
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