Effect of liver-specific miR-181c overexpression on lipogenesis.

Western blot (A) and quantitative polymerase chain reaction (qRT-PCR, B) of IDH1 in the liver from AAV-8 vector-injected mice. Western blot (C) and qRT-PCR (D) of IDH2 in the liver of AAV-8 vector-injected mice. (E) qRT-PCR was used to assess liver mRNA expression of sterol regulatory element binding transcription factor 1 (SREBP1), fatty acid synthase (FASN), and ATP citrate lyase (ACLY) genes, which are involved in lipogenesis. OE, overexpression; Scr, scrambled oligonucleotide. Data are expressed as mean ± SEM; n = 8 per group. * p<0.05 by t-test analysis. *p<0.05, **p<0.01 by two-sample t-test.