ppat.1010134.g001.tif (458.1 kB)
Effect of actG on bacterial growth, biofilm formation, and water-insoluble EPS synthesis in S. mutans.
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posted on 2021-12-03, 18:29 authored by Qizhao Ma, Yangyang Pan, Yang Chen, Shuxing Yu, Jun Huang, Yaqi Liu, Tao Gong, Jing Zou, Yuqing Li(A) Growth curves of S. mutans UA159, UA159 pDL278, UA159 pDL278-actG, and UA159 ΔactG in anaerobic condition for 12 h. (B, C) The biofilm biomass was determined by crystal violet staining assay (B) and anthrone-sulfuric acid method (C) when cultured in BHIS (1% sucrose wt/vol) in anaerobic condition for 6h, 12h, and 24h, respectively. Results are presented as mean ± SD (* P < 0.05, ** P < 0.01 or *** P < 0.001).
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used mass spectrometryrat caries modeloverexpressing 15 katsinsoluble extracellular polysaccharidesfrequently occurring postframe deletion mutantdiv >< pcould negatively regulateclinically isolated strainsptm ), emerginginsoluble eps synthesisimportant virulence factoractg could increase>, providing insightsstreptococcus mutans mutans gtfs ),vitro virulence differencestranslational modificationtaken togetherstudy demonstratedspecifically transferresults showedprotein acetyltransferaseprevious studypossible substratespart explainedexpression levelenzymatic mechanismdirect intermediatescoa ).biofilm formationbiofilm biomassbacterial virulencealso demonstratedactg acetylate gtfs
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