pone.0246647.s002.tif (2.57 MB)
Effect of additives on TaqPath RT-qPCR.
figure
posted on 2021-02-03, 18:59 authored by Thomas G. W. Graham, Claire Dugast-Darzacq, Gina M. Dailey, Xammy H. Nguyenla, Erik Van Dis, Meagan N. Esbin, Abrar Abidi, Sarah A. Stanley, Xavier Darzacq, Robert TjianCq values are shown for 10 μL TaqPath reactions containing 5 x 104 molecules of in vitro-transcribed N gene RNA and 5 μL of the specified concentrations of various additives. TX100, Triton X-100. NP-40, Nonidet P-40. T20, Tween 20. ICA-630, Igepal CA-630. Sark, sarkosyl (sodium lauroyl sarcosinate). DTT, dithiothreitol.
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