pone.0243333.s004.tif (8.98 MB)

Effect of Triton X-100 on extraction-based and direct RT-qPCR protocol performed on a synthetic N transcript or a clinical sample.

figure

posted on 2021-04-14, 17:55 authored by Julien Fassy, Caroline Lacoux, Sylvie Leroy, Latifa Noussair, Sylvain Hubac, Aurélien Degoutte, Georges Vassaux, Vianney Leclercq, David Rouquié, Charles-Hugo Marquette, Martin Rottman, Patrick Touron, Antoinette Lemoine, Jean-Louis Herrmann, Pascal Barbry, Jean-Louis Nahon, Laure-Emmanuelle Zaragosi, Bernard Mari

A. In vitro-transcribed viral N gene was added to either a Triton X-100 containing lysis buffer (Tx) or to TE buffer (TE). The samples were heated or not at 65°C for 10 min. RNA extraction was performed or not (direct) and N1 or RNP levels were determined by RT-qPCR using the Biomark^TM -HD system. B. A similar protocol as in A was used but the starting material were two consecutive sample collection from the same patient processed either in a Triton X-100 containing lysis buffer or to TE buffer.

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