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Effect of IL-1β, tumor necrosis factor alpha (TNF-α) and IL-17 used alone and in combination on C-Jun translocation

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posted on 2011-12-31, 04:37 authored by Corinne Granet, Wova Maslinski, Pierre Miossec

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Taken from "Increased AP-1 and NF-κB activation and recruitment with the combination of the proinflammatory cytokines IL-1β, tumor necrosis factor alpha and IL-17 in rheumatoid synoviocytes"

Arthritis Research & Therapy 2004;6(3):R190-R198.

Published online 26 Feb 2004

PMCID:PMC416439.

Copyright © 2004 Granet et al., licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

Rheumatoid arthritis synoviocytes were stimulated for 30 min with cytokines at the cited concentrations. Cells were fixed in 4% paraformadehyde and immunofluorescence staining was performed. Results are representative of three independent experiments. Effects of proinflammatory cytokines used alone at low and optimal concentrations and in combination on C-Jun nuclear translocation after 30 min of exposure are shown.

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