Effect of DAP3 silencing on the expression of mitochondrial genes and metabolism in THP-1 macrophages.

(A) RT-qPCR analysis of relative DAP3 RNA levels in THP-1 macrophages with (shDAP3) and without (shNS) DAP3 silencing. The level of DAP3 RNA without silencing was arbitrarily defined as 1. (B) Immunoblot analysis of DAP3 in THP-1 macrophages with and without DAP3 silencing. (C) The relative RNA levels of mitochondrial genes, IL-1β and DAP3 in THP-1 macrophages with and without DAP3 silencing and with and without HBV treatment were determined by RT-qPCR. (D), (E) and (G) are the metabolic parameters of the OCR of THP-1 macrophages shown in Fig 3F–3H, respectively. (F) THP-1 macrophages with and without DAP3 silencing were treated with 1 μg/mL HBeAg for 48 hours and then subjected to Seahorse analysis for ECAR. N.S., not significant; *, p<0.05; **, p<0.01; ***, p<0.001.

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