ER-phagy and nucleophagy cargos not enclosed within autophagosomes accumulated in the cytosplasm of yep1Δ cells.

(A) Applying the degron protection assay on the cytosolic protein Pyk1-AID-mECitrine. Prior to observation, cells were treated with (+Ad-IAA) or without (−Ad-IAA) 5-adamantyl-IAA for 1.5 hours. BF, brightfield. Cpy1-mCherry is a vacuole lumen marker. Bar, 5 μm. (B) Applying the degron protection assay on Epr1-AID-mECitrine. BF, brightfield. Bar, 5 μm. (C) Applying the degron protection assay on Rtn1-AID-mECitrine. BF, brightfield. Bar, 5 μm. (D) Electron microscopy images of ER-phagy/nucleophagy cargo structures with filamentous membrane protrusions in yep1Δ cells. N, nucleus; V, vacuole. Pink arrows denote filamentous membrane protrusions that extend from the ER-phagy/nucleophagy cargo structures. Bar, 1 μm.

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