ppat.1009784.s002.tif (1.12 MB)
E248R and mutants, E199L binding to endogenous NPC1.
figure
posted on 2022-01-26, 18:38 authored by Miguel Ángel Cuesta-Geijo, Isabel García-Dorival, Ana del Puerto, Jesús Urquiza, Inmaculada Galindo, Lucía Barrado-Gil, Fátima Lasala, Ana Cayuela, Carlos Oscar S. Sorzano, Carmen Gil, Rafael Delgado, Covadonga AlonsoMembranes used to compose Fig 3B. Dashed boxes were taken to create the western blot composite figure. Membranes were revealed with mouse anti-GFP antibody, rabbit anti-NPC1, rabbit anti-NPC2 and mouse anti-GAPDH antibody.
(TIF)
History
Usage metrics
Categories
Keywords
yet essential processinfectious cycle startsdynamin mediated endocytosisviral nucleic acidsmaller viral factoriesnpc1 ko cellsinternalized via clathrinarrested within endosomeslysosomal membrane proteinsinner viral membranecellular endosomal proteinsasfv proteins e248rmembrane fusion stependosomal proteins mightendosomal membraneendosomal proteinsviral proteinslimiting membranefusion stependosomal exitviral fusionviral coresviral adsorptionasfv proteinsvirion dissolvingupon decapsidationresults suggestph actsouter capsidnpc1 knocknew insightsmultilayer structurelate endosomeincoming virionshost cellfindings paveendocytic pathwayasfv infectivityasf virionalso observed
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC