Distribution pattern of 6mA in the genomic DNA of A. ferrooxidans ATCC 23270.

(A) Heatmap of 6mA modifications in the genomic DNA of A. ferrooxidans ATCC 23270 under Fe²⁺ and RISCs oxidizing conditions. The genome methylation in the Fe²⁺ oxidizing conditions was mostly upregulated (in red) and, to a small extent, downregulated (in blue) compared to that in the RISCs oxidizing condition, while the genes within the group differed more. (B) Distribution pattern of 6mA peaks around the gene body in A. ferrooxidans ATCC 23270 under Fe²⁺ (top panel) and RISCs (bottom panel) oxidizing conditions. 6mA levels were greater in gene bodies than in intergenic regions under both conditions. (C) Distribution pattern of 6mA peaks around the TSS in A. ferrooxidans ATCC 23270 under Fe²⁺ (top panel) and RISCs (bottom panel) oxidizing conditions. 6mA levels decreased at the TSS, followed by a sharp increase immediately after the TSS under both conditions.