Distribution of activated macrophages over time in patients and controls following stimulation with SLA.

Monocyte derived macrophages from LCL (n = 20), healthy non endemic controls (n = 10) and healthy endemic controls (n = 20) were stimulated with L.donovani antigen (50μg/ml). Percentage of activated cells was determined at A) 24h, B) 48h and C) 72h by analyzing expression of CD14 and CD40 by flowcytometry. In the vertical scatter plot each symbol represents a different subject. Horizontal bars represent the median. For statistical analysis, nonparametric Kruskal-Wallis test followed the Dunn’s multiple comparison test was used was used (*p<0.05; **p<0.01; ***p<0.001).