pone.0261853.g004.tif (431.17 kB)
Distribution of Ct residuals relative to sample-specific average Ct values.
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posted on 2022-01-13, 18:44 authored by Margaret G. Mills, Emily Bruce, Meei-Li Huang, Jessica W. Crothers, Ollivier Hyrien, Christopher A. L. Oura, Lemar Blake, Arianne Brown Jordan, Susan Hester, Leah Wehmas, Bernard Mari, Pascal Barby, Caroline Lacoux, Julien Fassy, Pablo Vial, Cecilia Vial, Jose R. W. Martinez, Olusola Olalekan Oladipo, Bitrus Inuwa, Ismaila Shittu, Clement A. Meseko, Roger Chammas, Carlos Ferreira Santos, Thiago José Dionísio, Thais Francini Garbieri, Viviane Aparecida Parisi, Maria Cassia Mendes-Correa, Anderson V. de Paula, Camila M. Romano, Luiz Gustavo Bentim Góes, Paola Minoprio, Angelica C. Campos, Marielton P. Cunha, Ana Paula P. Vilela, Tonney Nyirenda, Rajhab Sawasawa Mkakosya, Adamson S. Muula, Rebekah E. Dumm, Rebecca M. Harris, Constance A. Mitchell, Syril Pettit, Jason Botten, Keith R. JeromeResiduals were consistent between laboratories regardless of viral load. Residuals were plotted against sample-specific average Ct values. Each colored dot represents a different participating laboratory per legend.
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viral transport mediapurified nucleic acidsgenerate actionable datafewer experimental resourcesless &# 8221oral pharyngeal samplesconcordance across laboratoriesaccessible qualitative methodless &# 8220direct &# 8221pcr assay methods&# 8220participating laboratoriesnegative samplesdirect rtresults suggestreliable detectionqpcr methodpotently inhibitpcr reactionobserved 100lower costhouse equipmentfeasible optionequipment shortagesefficient covidcorrect identificationcontain charcoalcommon reagentscommon protocol2 rna2 detection2 ).
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