pone.0298835.g006.tif (505.07 kB)
Differentially expressed miRNAs between CM and CF.
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posted on 2024-02-29, 18:34 authored by Dessie Salilew-Wondim, Michael Hoelker, Eva Held-Hoelker, Franca Rings, Ernst Tholen, Christine Große-Brinkhaus, Karl Shellander, Carina Blaschka, Urban Besenfelder, Vita Havlicek, Dawit TesfayeA) Volcano plot showing the average (log2 fold change) expression of miRNAs in CM compared to CF. Red and green dots represent the up and downregulated miRNAs, respectively in CM compared to the CF group. The black dots represent miRNAs that did not show any significant expression differences between CM and CF. B) The heatmap representing the expression patterns of differentially expressed miRNAs within and across the biological replicates. The red and green colors represent increased and decreased miRNA expression.
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total rna includingsexual dimorphic mirnamirna analysis showedmirdeep2 software packageedger bioconductor packageviable elongating embryosmaternal reproductive microenvironmentbovine elongated embryosbiased embryo lossesholsteins frisian cows19 mirnas includingelongated embryo samplesrecovered elongating embryosfemale embryos developedmale embryos developedelongated femalefemale embryosembryos developedmale embryosmaternal environmentfemale ratioxlink ">skewed malesex ratiosequence alignmentrecovery rateperformed usinghighly expressedgestation periodexpression levelelongation perioddone usingdifferentially expressedday 13cows comparedclear whetherbiochemical secretion6 %.4 mirnas254 mirnas05 ).
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