Differentially expressed genes.

(A) Volcano plots show differences in gene expression in the serum-cultured vs. broth-cultured conditions. Colored circles indicate significantly up- (red) and down-regulated (blue) genes (FDR < 1e^-5 and |fold change| > 1.5), which are also indicated by number (#). (B) Three-way Venn diagram illustrating the bacterial genes that were altered in the serum culture relative to the broth culture condition (1, 2 and 4 h). The findings indicate that 191 and 174 transcripts were consistently up- and down-regulated (FDR < 1e^-5 and |fold change| > 1.5), respectively, in serum compared to medium.