ppat.1011859.s005.tif (1.42 MB)
Determination of pathogen load in pupal fat body and distribution in ovarioles after RNAi.
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posted on 2023-12-07, 18:49 authored by Chunxia Wang, Bin Yu, Xianzhi Meng, Dan Xia, Boyan Pei, Xiangyou Tang, Guizheng Zhang, Junhong Wei, Mengxian Long, Jie Chen, Jialing Bao, Chunfeng Li, Guoqing Pan, Zeyang Zhou, Tian Li(A) Pathogen load in the fat body after RNAi Vg and RNAi GFP treatment. (B) Pathogen load in the fat body after RNAi VgR and RNAi GFP treatment. The pathogen load is represented by copies of Nb-β-tubulin. Bars represent the means ± SD of three independent experiments. ns, not significant. (C) The distribution of N. bombycis in the ovarioles after injection of dsVg, dsVgR and dsGFP. The arrowhead shows the infection site, N. bombycis spores were stained using FB28 (blue); proliferative N. bombycis was labelled with antibody conjugated to Alexa Fluor 488 (green); nuclei were stained using PI (red). OS, ovariole sheath; FC, follicular cell; O, oocyte; NC, nurse cell.
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von willebrand domainunknown function domainmolecular mechanisms underlyingmechanisms remain unknownmajor economic lossesearly pupal stagediv >< pbuilt large vacuolesinfecting follicular cellselucidated key aspectsinfecting nurse cellsunderlying tot processestot ); howeverthereby penetrating oocytesobligate intracellular parasiteshijacks host vitellogeninfound significant reductionsnosema bombycis bombyx mori restructures ovariole cellsnurse cellsmori bombycis key factorsn b tot processwhole processtypical microsporidiumtransovarial transmissionpathogen loadparasites restructuredparasites commencedovary wallovariole sheathinfect almostfarming industrydramatic decreasecrucial role
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