ppat.1011859.s003.tif (1.9 MB)

Detection of polyclonal antibodies prepared in this study.

figure

posted on 2023-12-07, 18:49 authored by Chunxia Wang, Bin Yu, Xianzhi Meng, Dan Xia, Boyan Pei, Xiangyou Tang, Guizheng Zhang, Junhong Wei, Mengxian Long, Jie Chen, Jialing Bao, Chunfeng Li, Guoqing Pan, Zeyang Zhou, Tian Li

(A) Detection of polyclonal antibodies against BmVg and BmVgR using western blotting. (B) Subcellular localization of BmVg and BmVgR in ovarioles. (B1) Localization of BmVg in uninfected ovarioles. (B2) Negative serum was used as the control. (B3) Localization of VgR in uninfected ovarioles. (B4) Localization of N. bombycis spores and BmVgR in oocytes. (C) Detection of polyclonal antibodies that recognize the N. bombycis spore wall protein 12, spore wall protein 26, and spore wall protein 30 using western blotting. The arrowhead shows N. bombycis; N. bombycis spores were stained using FB28 (blue); proliferative N. bombycis were labelled with rabbit polyclonal antibody against N. bombycis (Alexa488, green); BmVg was detected using a mouse anti-BmVg polyclonal antibody (Alexa647, yellow); nuclei were stained using PI (red). OS, ovariole sheath; FC, follicular cell; O, oocyte; NC, nurse cell.