pone.0255257.s004.tif (598.59 kB)
Detection of KMT2A-MLLT10 fusion.
figure
posted on 2021-07-23, 17:26 authored by Daichi Sadato, Chizuko Hirama, Ai Kaiho-Soma, Ayaka Yamaguchi, Hiroko Kogure, Sonomi Takakuwa, Mina Ogawa, Noriko Doki, Kazuteru Ohashi, Hironori Harada, Keisuke Oboki, Yuka Harada(A) The fusion sequence detected by targeted RNA sequencing is shown. Arrows indicate the primers for amplifying the target region. (B) A fusion gene confirmed by RT-PCR is shown. The following parameters were used with the PrimeSTAR GXL DNA Polymerase (TAKARA): 98°C for 3 min, followed by 35 cycles at 98°C for 10 s, 70°C for 15 s, and 68°C for 30 s. The sample from Patient#176 was used as a negative control.
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