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pgen.1009686.s001.eps (2.34 MB)
Detection of TUBL protein expression in epidermal lysates by MS-based MRM analysis.
figure
posted on 2021-08-05, 17:27 authored by Akihiro Nita, Akinobu Matsumoto, Ronghao Tang, Chisa Shiraishi, Kazuya Ichihara, Daisuke Saito, Mikita Suyama, Tomoharu Yasuda, Gaku Tsuji, Masutaka Furue, Bumpei Katayama, Toshiyuki Ozawa, Teruasa Murata, Teruki Dainichi, Kenji Kabashima, Atsushi Hatano, Masaki Matsumoto, Keiichi I. NakayamaExtracted ion chromatograms are shown for TUBL-derived peptides in MRM analysis. MRM analysis detected two different TUBL-derived peptides with the amino acid sequences AQLVGQGVSSWR (A) and DTLSDLR (B). “Standard” indicates signals for the internal standard, a stable isotope–labeled recombinant TUBL protein expressed in and purified from bacteria. “Endogenous” indicates signals for peptides derived from endogenous TUBL in epidermal lysates prepared from Tubl+/+ or Tubl−/− mice. The purple vertical lines in the Endogenous chromatograms indicate the retention time of each endogenous TUBL peptide.
(EPS)
