pntd.0009400.s002.tif (4.16 MB)
Detection of immunoglobulin G (IgG) antibodies against Hantaan virus (HTNV) from Apodemus chejuensis sera and heart fluids by indirect immunofluorescence assay (IFA).
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posted on 2021-05-12, 17:45 authored by Kyungmin Park, Won-Keun Kim, Seung-Ho Lee, Jongwoo Kim, Jingyeong Lee, Seungchan Cho, Geum-Young Lee, Jin Sun No, Keun Hwa Lee, Jin-Won SongHTNV-infected Vero E6 cells were fixed on each slide. The sera (1:32 dilution) or heart fluids (1:2 dilution) of rodents were used to detect anti-HTNV IgG by IFA, Ac18-6, Ac18-17, Ac19-6, Ac20-30, and Ac20-31; heart fluids of Ac20-5 and Ac20-6. Positive and negative controls used were serum (Aa18-185) and phosphate buffered saline (PBS), respectively. Ac, A. chejuensis; Aa, A. agrarius.
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hemorrhagic feverApodemus agrarius chejuensisKorea Background OrthohantavirusesRNAgenotypetranscription-polymerase chain reactionHFRSJeju Islandorthohantaviruamplicon-based next-generation sequ...HTNV genomic sequencesetiological agentROKorthohantavirusehantavirus cardiopulmonary syndromeimmunofluorescence assay test
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