Description of the STAMP method.

From multi-channel videos of ToC co-cultures, the cancer cells (pre-stained in red) are localized and tracked in the red channel. The dying cancer cells (becoming green because of the caspase reporter) are identified in the green channel, after signal normalization and thresholding. The method monitors cancer cell deaths both in time and in space. The death signal is modeled to vanish during T time frames. Then, the spatiotemporal features of all death signals are integrated in a unique map, from which a parameter, named potential of death induction (P_death), is computed over time (see Materials and Methods for details).