Derepression of stress genes in cbf11Δ cells is associated with H3K9 hyperacetylation in their promoters (the second replicate) but not with altered total cellular acetyl-CoA levels.

(A) Average gene profiles of total H3 (bottom panels) and acetylated H3K9 (top panels) occupancy at stress-gene regions in WT and cbf11Δ cells, respectively. Genes upregulated as part of the core environmental stress response (CESR-UP, [9]) have been divided into those showing upregulation in untreated cbf11Δ cells (blue; n = 94, [15]) and the rest (red; n = 441). Average profile of all fission yeast genes is also shown for comparison (black; n = 6952). The curves represent mean RPM (reads per million mapped reads) values ± SEM. The promoter region is shaded. TSS—transcription start site. (B) Total cellular acetyl-CoA levels in WT, ppc1-537, cbf11Δ and Pcut6MUT cell extracts were determined by LC-MS. Mean and SD values of four independent replicates for ppc1-537 and five independent replicates for all other strains tested are shown. Two-sided Mann-Whitney U test was used to determine statistical significance.

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