ppat.1009425.s003.tif (241.41 kB)
Deletion of the tatABC genes does not influence pqsR expression.
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posted on 2021-08-30, 17:38 authored by Eliza Ye-Chen Soh, Frances Smith, Maxime Rémi Gimenez, Liang Yang, Rebecca Munk Vejborg, Matthew Fletcher, Nigel Halliday, Sophie Bleves, Stephan Heeb, Miguel Cámara, Michael Givskov, Kim R. Hardie, Tim Tolker-Nielsen, Bérengère Ize, Paul WilliamsThe data show that there are no differences in the expression of a chromosomal CTX::pqsR’-luxCDABE fusion in the P. aeruginosa wild type compared with the ΔtatABC mutant Data are presented as maximal light output as a function of growth (RLU/OD600). Experiments were repeated in triplicate at least twice.
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wild type levelsreduced bioluminescence revealedreduced antibiotic tolerancedependent quorum sensingdependent autoinduction resultingdiv >< pdefective biofilm architecture>< sub ><primary signalling defectidentify factors requiredpseudomonas aeruginosa tat prevented pqs>< sub xmlnsedna deficient biofilmsaeruginosa tat aeruginosa dependent qsp biofilm maturationtat tat substratesub xmlnsrieske sub>rhla pqse pqsa pqs phza1 lux cytc cytb 1 transposon librarysulfur subunitrieske ironqs ).parent strainmembrane vesiclemajor constituentgenetic complementationgenes resultsextracellular matrixextracellular dnaexogenous pqsenhanced susceptibilitydriven autoarginine translocationalso phenocopied34 validated
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