ppat.1009425.s007.tif (1.35 MB)
Deletion of petA in P. aeruginosa PA14 results in a reduction in the eDNA content of biofilms.
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posted on 2021-08-30, 17:38 authored by Eliza Ye-Chen Soh, Frances Smith, Maxime Rémi Gimenez, Liang Yang, Rebecca Munk Vejborg, Matthew Fletcher, Nigel Halliday, Sophie Bleves, Stephan Heeb, Miguel Cámara, Michael Givskov, Kim R. Hardie, Tim Tolker-Nielsen, Bérengère Ize, Paul WilliamsBiofilms of wild type PA14, the ΔtatABC and ΔpetA mutants and the genetically complemented PA14 mutant (ΔpetA::(CTX::petA)) were grown cultured statically and stained for eDNA with YOYO-1. (A) confocal fluorescence microscopy images and (B) eDNA quantification. Experiments were repeated in triplicate at least twice. ***p < 0.001, **p < 0.01.
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wild type levelsreduced bioluminescence revealedreduced antibiotic tolerancedependent quorum sensingdependent autoinduction resultingdiv >< pdefective biofilm architecture>< sub ><primary signalling defectidentify factors requiredpseudomonas aeruginosa tat prevented pqs>< sub xmlnsedna deficient biofilmsaeruginosa tat aeruginosa dependent qsp biofilm maturationtat tat substratesub xmlnsrieske sub>rhla pqse pqsa pqs phza1 lux cytc cytb 1 transposon librarysulfur subunitrieske ironqs ).parent strainmembrane vesiclemajor constituentgenetic complementationgenes resultsextracellular matrixextracellular dnaexogenous pqsenhanced susceptibilitydriven autoarginine translocationalso phenocopied34 validated
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