DGAT1 knockdown increases nephrocyte lipid peroxidation.

(A) Confocal panels represent ratio of oxidised (500- to 540-nm emission) to non-oxidised (570- to 610-nm emission) forms of the lipid peroxidation sensor BODIPY 581/591 C11 in pericardial nephrocytes (dotted outlines) from STD larvae and HFD larvae carrying Dot>DGAT1[i] or Dot>ATGL. (B) Graph quantifies oxidised:non-oxidised ratios of the lipid peroxidation sensor BODIPY 581/591 C11 in pericardial nephrocytes for the dietary and genetic manipulations in A. On HFD, lipid peroxidation is increased by DGAT1 knockdown but not by ATGL expression. See S1 Data for details of p-values and the type of statistical model used for all graphs in this study. S2 Data provides the source data used for all graphs and statistical analyses. ATGL, adipose triglyceride lipase; DGAT1, diglyceride acyltransferase 1; HFD, high-fat diet; STD, standard diet.

(TIF)