DAF does not affect replication of PR8, PR8-HK4,6, PR8-HK4 and PR8-HK6.
A: Measurement of viral plaques diameter after infection of MDCK cells monolayers. Data shown as mean±sd from two independent experiments, each corresponding to six independent infections for each virus. Each point represents an individual plaque. B-E: Replication kinetics of A/Puerto Rico/8/1934 (PR8) (B), A/X-31 (PR8-HK4,6) (C), PR8 containing the segment 4 of A/Hong Kong/1/68 (HK68) (PR8-HK4) (D) and PR8 containing the segment 6 of HK68 (PR8-HK6) (E) in mouse embryonic fibroblasts (MEFs) derived from C57BL/6J WT or Daf-/- mice at multiplicity of infection (MOI) = 0.005. Data shown as mean±SEM, from two independent experiments. Statistical analysis detailed in materials and methods. F, G: Cell death of primary lung cells derived from WT or Daf-/- mice infected or mock-infected with PR8, PR8-HK4,6, PR8-HK4 or PR8-HK6 and treated with serum (F) or heat-inactivated control (G). Results are expressed as mean±sd from three replicates from two independent experiments. Statistical analysis detailed in materials and methods.
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