ppat.1010219.s002.tif (1.12 MB)

Cytotoxicity data of itaconate, DI, and 4OI on dTHP1 and A549 cells.

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posted on 2022-01-13, 18:46 authored by Aaqib Sohail, Azeem A. Iqbal, Nishika Sahini, Fangfang Chen, Mohamed Tantawy, Syed F.H. Waqas, Moritz Winterhoff, Thomas Ebensen, Kristin Schultz, Robert Geffers, Klaus Schughart, Matthias Preusse, Mahmoud Shehata, Heike Bähre, Marina C. Pils, Carlos A. Guzman, Ahmed Mostafa, Stephan Pleschka, Christine Falk, Alessandro Michelucci, Frank Pessler

Cytotoxicity was determined by applying increasing concentrations of the compounds to dTHP1 (25 x10³ cells/well) and A549 cells (20 x10³ cells/well) and measuring cell respiration with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay after 24 h. Curves for dTHP1 and A549 cells are shown in the same graphs for comparison, illustrating that itaconate and 4OI tend to be more toxic on dTHP1 cells, whereas DI is more toxic on A549 cells. A. Itaconate (n = 3 experiments, each with n = 8 replicates). B. DI (n = 3 experiments, each with n = 8 replicates). C. 4OI (n = 1 experiment with n = 8 replicates). Means ±SEM. D. Table summarizing the concentrations at which mean viability was reduced by 5, 10, 20 or 50%. The concentrations used in this study were selected to be < CC05. CC = concentration at which a given percentage of cells was non-viable.

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