pone.0246647.s003.tif (3.23 MB)
Cytopathic effect (CPE) assay for inactivation of SARS-CoV-2.
figure
posted on 2021-02-03, 18:59 authored by Thomas G. W. Graham, Claire Dugast-Darzacq, Gina M. Dailey, Xammy H. Nguyenla, Erik Van Dis, Meagan N. Esbin, Abrar Abidi, Sarah A. Stanley, Xavier Darzacq, Robert TjianInactivation experiments were performed in water or Solution 2 (10 mM Tris, pH 8, 1 mM EDTA, 0.5% Tween 20, 0.5 mM DTT), with or without proteinase K. Images were taken 3 days after inoculation of Vero E6 cells with no virus (i), untreated virus (ii), or virus incubated for 30 min at 37°C and 30 min at 75°C in the indicated solutions (iii-vi). Cytopathic effect (CPE) was visible in cultures inoculated with active virus (ii) but not in cultures inoculated with heat-inactivated virus (iii-vi).
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