Cytokine profiles induced by in vitro infection of MyD88^+/+ and MyD88^-/- LP cells from noninfected mice.

(A) Naïve LP cells were cultured ex vivo with STAg or infected with a type I strain (RH) or type II strain (PTG) of T. gondii at the indicated MOI. Supernatants were collected after 72 hrs and IL-12p40 was quantified by ELISA. Values are the means ± SEM of three independent experiments. (B) Similarly, supernatants from cps1-1 and cps1-1Δgra24 infected MyD88^+/+ and MyD88^-/- LP cells were collected to quantify IL-12p40 production by ELISA. (C) Naïve WT LP cells were infected with cps1-1 or cps1-1Δgra24 tachyzoites (MOI 1:1) and supernatants were harvested at 72 hours to analyze secreted immune factors using a mouse cytokine/chemokine proteome array. An unpaired Student’s t test was used to compare infected WT and KO responses where ***p<0.001.

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