Coomassie stained SDS-PAGE and Western blot analysis of Schistosoma haematobium tetraspanins.

1μg of each TSP was electrophoresed on SDS-PAGE and Comassie-stained: Protein ladder (A1), Sh-TSP-2 (A2), Sh-TSP-4 (A3), Sh-TSP-18 (A4), Sh-TSP-5 (A5), Sh-TSP-23 (A6) and Sh-TSP-6 (A7). Western blot of TSPs using an anti-His monoclonal antibody: Protein ladder (B1), Sh-TSP-2 (B2), Sh-TSP-4 (B3), Sh-TSP-18 (B4), Sh-TSP-5 (B5), Sh-TSP-23 (B6) and Sh-TSP-6 (B7). The expected size of recombinant Sh-TSP-2 was 12.4 kDa, since it was expressed without a tag. For Sh-TSP-4, Sh-TSP-5, Sh-TSP-6, Sh-TSP-18 and Sh-TSP-23, since they contained the TRX-tag from the cloning vector, their expected size ranged from ~28 kDa to ~38 kDa.

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