pone.0247963.g002.tif (1.09 MB)
Conjugation of SpyCatcher-VLPs and -NPs.
figure
posted on 2021-03-04, 18:59 authored by Alexander A. Cohen, Zhi Yang, Priyanthi N. P. Gnanapragasam, Susan Ou, Kim-Marie A. Dam, Haoqing Wang, Pamela J. BjorkmanA. SpyCatcher-AP205-VLP conjugations with SpyTagged-HA trimers (without the Y98F substitution). B. Purification of conjugated SpyCatcher-VLPs. Left: SEC separation of conjugated VLPs from free HA trimers. Right: Reducing SDS-PAGE analysis of VLPs and purified HAs. C. SpyCatcher-mi3 NP conjugations with SpyTagged-HA Y98F trimers. D. Purification of homotypic SpyCatcher-NPs. Left: SEC separation of conjugated NPs from free HA trimers. Right: Reducing SDS-PAGE analysis of NPs and purified HAs. E. Purification of heterotypic mosaic NPs. Left: SEC separation of conjugated NPs from free HA trimers, including SEC profile of purified conjugated NPs after one month storage at 4 ˚C. Right: Reducing SDS-PAGE analysis of NPs and purified HAs.
