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Conjugation of SpyCatcher-VLPs and -NPs.

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posted on 2021-03-04, 18:59 authored by Alexander A. Cohen, Zhi Yang, Priyanthi N. P. Gnanapragasam, Susan Ou, Kim-Marie A. Dam, Haoqing Wang, Pamela J. Bjorkman

A. SpyCatcher-AP205-VLP and SpyCatcher-mi3 conjugations with SpyTagged-HA trimers. B. Purification of homotypic and mosaic SpyCatcher-VLPs and SpyCatcher-mi3s. Left: SEC separation of conjugated NPs from free HA trimers. Right: Reducing SDS-PAGE analysis of NPs and purified HAs.

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