Complementation test of the dominant-negative FtsW variants isolated by the wrinkled-colony-based screen.

Plasmids pDSW208, pSEB429 (pDSW08, P₂₀₄::ftsW) or its derivatives harboring a dominant-negative ftsW mutation were transformed into strain SD237 [W3110, leu::Tn10 ftsW::kan/pDSW406 (pBAD33, P_BAD::ftsW)] on LB plates with ampicillin and 0.2% arabinose. The next day, a single transformant of each resulting strain was resuspended in 1 ml of LB medium, and serially diluted ten-fold. 3 μl of each dilution was spot on LB plates with antibiotics, with or without increasing concentrations of IPTG. Plates were incubated at 30°C overnight and imaged.

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