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Complementation test of the FtsW variants generated by site-directed mutagenesis.

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posted on 2022-01-05, 18:35 authored by Ying Li, Adrien Boes, Yuanyuan Cui, Shan Zhao, Qingzhen Liao, Han Gong, Eefjan Breukink, Joe Lutkenhaus, Mohammed Terrak, Shishen Du

Transformation and spot tests were performed as in S1 Fig.

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    PLOS Genetics

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    substrate lipid iiclass b penicillinxlink "> sedsseds protein criticalwidely conserved peptidoglycanmodeled ftsw structurebacterial cell growthaffect ftsw localizationseptal pg synthesispotential active siteseds proteins recognizeactive siteseds proteinsseds familyhighly conservedbacterial morphogenesisbacterial cytokinesistranspeptidase activitytaken togethersynthesize pgscattered aroundresults suggestresidues correspondingpromising targetspg layernew antibioticsnegative allelesmay aidglycan strandsform complexesextracellular loopscrucial rolescentral cavitybuilding blockbinding proteins

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