Complementation of R. equi ΔvapA with pVapA-ST and pVapA::VapD-ST.

Murine J774A.1 cells were infected with either R. equi ΔvapA or R. equi ΔvapA carrying either pVapA-ST or pVapA::VapD-ST. Intracellular R. equi were enumerated 0,24 and 48 hours post infection. Intracellular growth is shown as fold changes relative to time zero. Error bars represent the mean and standard deviation. Horizontal lines show the Dunnet’s T3 adjusted P value corrected for multiple comparisons. For simplicity only relevant comparisons are shown.