Comparison of non-neutralizing antibodies against N611 epitopes on day of challenge.

Serum neutralizing activity on the day of challenge (week 84) was evaluated against the parental BG505 and N611A mutant PVs using the TZM-bl assay for all but one immunized RM (n = 29). The log10 increase in ID₅₀ titer of the N611A mutant compared to the parental BG505 Env is shown in vertical plots for each RM grouped by vaccination, challenge outcome, and titer/challenge outcome (A—C). Red symbols indicate HVV + SOSIP immunized RM; black symbols indicate SOSIP immunized RM. (A) vaccination groups HVV + SOSIP vs SOSIP (p>0.05), (B) protected vs infected (p = 0.047), and (C) high titer protected vs. low titer protected (p = 0.006) and low titer infected (p = 0.004) are shown. Mann-Whitney tests were used to perform the two group comparisons in (A) and (B) and a Kruskal-Wallis test with Dunn’s correction was used for the three-group comparison in (C). All were performed using GraphPad Prism (*p<0.05, **p<0.01). Horizontal bars in (A—C) represent the median of the group. The dashed lines indicate the threshold of 0.5log10 fold increase over the parental BG505.

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