Comparison of Borrelia killing using neutrophils from WT and PGLYRP1^-/- mice.

Neutrophils isolated from mouse bone marrow were incubated with 1x10⁵ B. burgdorferi for 1 hour in DMEM media in the absence (A) or presence of 10% mouse serum (B). The assay was performed in 96-well flat-bottom plates and volume was 50 μl. After 1 hour, 250 μl of BSK-H medium was added to the wells. The plates were incubated in microaerophilic conditions at 33°C for 72 hours. The Borrelia numbers were counted in Neubauer chamber under the dark-field microscope. The experiment was performed in triplicates and results from one independent experiment are shown. The bars represent mean ± SEM and p-values were determined by the Mann-Whitney test.

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