Comparison of frozen, dried, and fixed/paraffin embedded tissue for human lenses.

(A) Diagonal slice overlays show a shift to higher frequency and less intensity as the lens tissue goes from the most polar (frozen, rehydrated in buffer) to most nonpolar (paraffin wax) environment. Normalized to juvenile lens tissue peak at 1632 cm^-1 for frozen, 1636 cm^-1 for dried, and 1641 cm^-1 for fixed lens tissue. (B) Peak frequency distributions are heterogeneous, with distinct shifts to higher frequencies as the lens tissue goes from a polar to nonpolar environment. Red: Cataract lens tissue (frozen, then rehydrated in buffer); Blue: Juvenile lens tissue (frozen, then rehydrated in buffer); Purple: Cataract lens tissue (frozen, then dried under nitrogen); Yellow: Juvenile lens tissue (frozen, then dried under nitrogen); Light Blue: Cataract lens tissue (fixed and paraffin embedded); Green: Juvenile lens tissue (fixed and paraffin embedded).

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