Comparison of frozen and fixed tissue for mouse lenses.

(A) Diagonal slice overlays show a shift to higher frequency and less intensity as the lens tissue goes from the most polar (frozen, rehydrated in buffer) to most nonpolar (paraffin wax) environment. Normalized to wild type mouse lens tissue (peak at 1632 cm^-1 for frozen, 1641 cm^-1 for fixed). (B) Photographs of fixed, paraffin embedded lens slices shows a lens slice rejected from measurement because of large rips (top left, from Cryab-mutant lens sample 3) and a lens slice typical of those used in this study (bottom right, from Cryab-mutant lens sample 1). Maroon: Cryab-R120G mutant mouse lens tissue (frozen, then rehydrated in buffer); Green: wild type mouse lens tissue (frozen, then rehydrated in buffer); Red: Cryab-R120G mutant mouse lens tissue (fixed and paraffin embedded); Blue: wild type mouse lens tissue (fixed and paraffin embedded).

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