Clinical outcome and early myeloid cellular responses in BAFF-RFP^+/- mice after S. typhimurium infection.

WT mice and BAFF-RFP^+/- mice were infected i.p. with 500 CFU S. typhimurium. (A) Clinical scores and mouse weights were taken daily. (B) Spleen weights and splenic bacterial burdens were measured at day 3 and 4 p.i. Data are combined from three independent experiments (n = 6–10). (C and D) Cells from BMs and spleens were harvested at day 3 and 4 p.i., and myeloid cell subsets were determined by flow cytometry. (C) Numbers of BM Nphs (CD11b⁺Ly6G^hiLy6C^intSSC^int-), Pre DCs (CD11b⁺CD11c⁺Ly6C^-CD115⁺CX3CR1^hiCCR2⁺ MHCII⁺Ly6G^-SSC^-) and Ly6C^hi MOs (CD11b⁺CD11c^-CD115⁺CX3CR1⁺CCR2^hi MHCII^-Ly6G^-SSC^-) from BAFF-RFP^+/- mice after S. typhimurium infection. Graphs summarize data from three independent experiments (n = 6–8). (D) Numbers of splenic Nphs (CD11b^hiLy6G^hiLy6C^int SSC^int-NK1.1^-), cDCs (CD11c^hiCD8^+/-B220^-Ly6G^-NK1.1^-) and Ly6C^hi MO/DCs (CD11b^hi Ly6C^hiCD11c^hi/-SSC^-Ly6G^-NK1.1^-) from BAFF-RFP^+/- mice after S. typhimurium infection. Data are combined from four independent experiments (n = 6–12). (A) Timeline data of clinical scores and mouse weights were analyzed by Two-Way ANOVA with Holm-Sidak’s multiple comparison test. (B-D) Bar graphs show means ± SEM; * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001, as determined by One-Way Anova with Holm-Sidak multiple comparisons test.