Ciliation time course and cilia length for immortal cell lines.

A) Proportion of ciliated cells at 0, 8, 16, 24, 48, 72, and 96 hours post serum starvation. We performed three replicates for each time point, with error bars denoting the range for each time point across three batches. The numbers of cells counted at each time point and in each batch are listed in the table below the graph. B1 = batch 1, B2 = batch 2, and B3 = batch 3. B) Cilium length across time points. Black bars in each column represent median length. Significant p-values are denoted as follows: 0.01 to 0.05 (*), 0.001 to 0.01 (**), and <0.001 (***). No *’s are shown for differences that are not statistically significant. C) Proportion of ciliated cells without and with stimulation. Each symbol represents the proportion of ciliated cells in one batch. No differences between untreated and treated cells within each cell line were statistically significant using the Mann-Whitney test. D) Cilium length without stimulation and with stimulation. Large symbols = median cilium length within a batch. Small symbols = cilium length of one cilium. One coverslip was imaged for each cell line and time point. The total number of cells counted across three batches are listed below each column of the graphs.