Chronological Lifespan Assay

Figure 1. Chronological Lifespan (CLS) of the Hermes Mutants. CLS assays monitor the continuous decline in viability without detectable regrowth in a cell culture over time. Illustrated, CLS survival curves of two, independent Hermes mutant pools in YES media. Data for the wild type control was obtained from Rallis et al. (2013).

Figure 2. Gene-Based Ageing Analyses. Top, analysis v1.0 uses the number of unique insertion counts/sites within a gene and the overall 10 kb region surrounding it. Bottom, analysis v2.0 uses the average and mean unique insertion counts/sites of 2n kb gene windows where n = 500 nt, 1 kb and 1.5 kb for 1, 2 and 3 kb gene windows respectively. For both analyses, a gene was defined as a whole, therefore, it was set to include untranslated regions, introns, and exons, rather than just the coding sequences.