ppat.1009706.s006.tif (25.87 MB)
Chloroquine does not affect RBD uptake and minimally affects endosomal acidification in AGS cells.
figure
posted on 2021-07-12, 17:46 authored by Chaitra Prabhakara, Rashmi Godbole, Parijat Sil, Sowmya Jahnavi, Shah-e-Jahan Gulzar, Thomas S. van Zanten, Dhruv Sheth, Neeraja Subhash, Anchal Chandra, Akshatha Shivaraj, Patricia Panikulam, Ibrahim U, Vijay Kumar Nuthakki, Theja Parassini Puthiyapurayil, Riyaz Ahmed, Ashaq Hussain Najar, Sai Manoz Lingamallu, Snigdhadev Das, Bhagyashri Mahajan, Praveen Vemula, Sandip B. Bharate, Parvinder Pal Singh, Ram Vishwakarma, Arjun Guha, Varadharajan Sundaramurthy, Satyajit MayorA, B: Using AGS cells treated with Control or CQ for 12 hours, RBD, dextran and transferrin uptake experiment (A) and FITC/TMR endosomal ratio estimation experiment (B) was conducted. Quantification in G and H show that RBD uptake, dextran uptake and FITC/TMR endosomal ratio are unaffected by long term treatment with CQ. Number of cells > 80 for each condition. Data representation is as described in Fig 1.
(TIF)
History
Usage metrics
Categories
Keywords
pH-dependent endocytic pathwayuptakeinhibitionNH 4 ClNiclosamideACE 2. Ectopic expressionattenuates Spike-pseudovirus entryEndosomal acidification inhibitorsCLICSARS-CoV -2SARS-CoV -2 spike proteinBafilomycinA 1endosomal pHCGinfectiontarget SARS-CoV -2 entryAGS-ACE 2 cellsSARS-CoV -2 entry inhibitorreceptor binding domainRBDhost endo-lysosomal network
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC